RNA-seq of mimic miR-34a transfected H1299 and DDX21 KO cell lines.

In order to investigate the role of DDX21 in RNA activation by miR-34a, next-generation sequencing analysis of RNA extracted from miR-34a transfected H1299 and DDX21 knock-out cell line was performed. Overall design: RNA profiles were generated by deep sequencing, using Illumina HiSeq 4000.

为探究DDX21在微小RNA-34a（miR-34a）介导的RNA激活过程中所发挥的作用，本研究对转染了miR-34a的H1299细胞系以及DDX21基因敲除细胞系中提取的RNA开展了下一代测序（next-generation sequencing）分析。实验整体设计：采用Illumina HiSeq 4000平台进行深度测序（deep sequencing）以生成RNA表达谱。