MBD-R2_Q2567.S2 ChIP-chip

modENCODE_submission_946 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody MBD-R2 Q2567

modENCODE_submission_946 本提交项源自Gary Karpen负责的modENCODE项目。如需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在确定黑腹果蝇（Drosophila melanogaster）全基因组中125种染色体蛋白质与组蛋白修饰的定位信息。所研究的蛋白质及修饰参与DNA复制、基因表达、基因沉默与遗传等核心染色体生物学功能。本研究将采用基因组芯片染色质免疫共沉淀（Chromatin ImmunoPrecipitation，ChIP）技术，初步利用三种细胞系与两个胚胎阶段的染色质开展定位分析，后续将针对部分蛋白质的分析拓展至另外四种动物组织/发育阶段。关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
关键词：ChIP-chip
实验类型：ChIP-chip
生物来源：细胞系：S2-DRSC；组织：胚胎来源细胞系；发育阶段：晚期胚胎阶段；性别：雄性；重复次数：4；实验因素：细胞系S2-DRSC；抗体：MBD-R2 Q2567