Isoenzyme equipment of lactate dehydrogenase (LDH), glutamate oxaloacetate transaminase (GOT) and malate dehydrogenase (MDH) of pyruvate-low PC-3 and DU145 cells as measured by free flow isoelectric focusing.

The percentual amounts were calculated as area under the curve (AUC) in percent of the respective total enzyme activity loaded onto the focusing column. Cyto = cytosolic isoenzyme, mito = mitochondrial isoenzyme, prec = cytosolic precursor of the mitochondrial isoenzyme. Unpaired Student’s t-test was performed to assess significancy. Mean ± SEM. n = 3. P-values (PC-3 vs DU145): LDH H-type and M-type (****)本研究中的百分比含量以加载至聚焦柱的对应总酶活性的百分比形式的曲线下面积（AUC）计算得到。其中，Cyto指代胞质同工酶（cytosolic isoenzyme），mito指代线粒体同工酶（mitochondrial isoenzyme），prec指代线粒体同工酶的胞质前体（cytosolic precursor of the mitochondrial isoenzyme）。本研究采用非配对Student t检验评估统计学显著性。数据以均值±标准误（Standard Error of the Mean, SEM）表示。每组样本量n=3。在PC-3细胞与DU145细胞的比较中，乳酸脱氢酶（Lactate Dehydrogenase, LDH）H型与M型的P值标注为(****)。