Dynamic and stable enhancer-promoter contacts regulate terminal differentiation [RNA-Seq]. Homo sapiens

Genome-wide chromosome conformation capture (Hi-C) and promoter-capture Hi-C (CHi-C) were performed during epidermal differentiation. These data indicate that dynamic and constitutive enhancer-promoter contacts combine to control gene induction during differentiation and that chromosome conformation enables discovery of new TFs with distinct roles in this process. Overall design: RNA-seq data from epidermal keratinocyte progenitor and differentiated cells.

本研究在表皮分化过程中开展了全基因组染色体构象捕获（Hi-C）与启动子捕获Hi-C（CHi-C）实验。所得数据表明，动态型与组成型增强子-启动子相互作用可协同调控分化进程中的基因诱导事件，且染色体构象分析能够助力发现在该过程中发挥独特功能的新型转录因子（TFs）。实验整体设计：涵盖表皮角质形成细胞祖细胞与分化细胞的RNA测序（RNA-seq）数据。