The transcriptional repressor Blimp-1/PRDM1 restrains Type III IFN signalling in mammary epithelial cells. Mus musculus

Expression profiling of wild-type and Prdm1 null mouse MECs using Illumina whole genome mouse V2 arrays. The hypothesis tested was that Prdm1-regulated gene expression in MECs is required for correct epithelial function. Overall design: Tamoxifen inducible Prdm1 null (Prdm1BEH/CA;ROSA26:CreERT2) and wild type MEC were treated with 4OH Tamoxifen on D0 of cuture. Total RNA was obtained from three wild type (C57BL6) and three Prdm1 null MECs each on D3 and D4 of culture and hybridized to Illumina WG6_V2 arrays

使用Illumina全基因组小鼠V2芯片，对野生型与Prdm1敲除型（Prdm1 null）小鼠乳腺上皮细胞（mouse mammary epithelial cells, MECs）开展基因表达谱分析。本实验所验证的科学假说为：Prdm1调控的乳腺上皮细胞基因表达，对于维持上皮细胞的正常生理功能是必需的。实验设计：将他莫昔芬诱导型Prdm1敲除（Prdm1BEH/CA;ROSA26:CreERT2）与野生型乳腺上皮细胞，于培养第0天（D0）经4-羟基他莫昔芬（4OH Tamoxifen）处理。分别于培养第3天（D3）与第4天（D4），从3株野生型（C57BL6）与3株Prdm1敲除型乳腺上皮细胞中提取总RNA，随后将其与Illumina WG6_V2芯片进行杂交。