Multiple actin regulatory proteins localize to phagocytic teeth (images for Figure 5 and Figure 5 Supplement)

RAW macrophages were transfected with fluorescently tagged actin binding proteins and challenged to ingest DAAMPs (11 mm, 1.4 kPa) functionalized with IgG and AF647-Cadaverine to assess localization to actin teeth (yellow arrowheads). Images were collected by laser scanning confocal microscopy.

将RAW巨噬细胞转染荧光标记的肌动蛋白结合蛋白（actin binding proteins），随后使其吞噬经免疫球蛋白G（IgG）与AF647-尸胺（AF647-Cadaverine）功能化修饰的DAAMPs（11 mm，1.4 kPa），以评估其向肌动蛋白齿（actin teeth）的定位情况（以黄色箭头标示）。图像通过激光扫描共聚焦显微镜（laser scanning confocal microscopy）采集。