Post-antibacterial effects of artonin E and streptomycin in <i>Staphylococcus aureus</i> ATCC 25923 and MRSA BAA-1720.

Bacterial growth inhibition was expressed in mm and measured using the disc diffusion assay. Test was performed in triplets. The mean and standard deviations are provided indicating the inhibition diameter. The MIC/MBCs of artonin E were similar for both S. aureus strains, and comparable MIC and MBCs were obtained using streptomycin. Minimum inhibitory concentration indicates that S. aureus ATCC 25923 was not grown after exposure to 3.9 μg/mL MIC of artonin E or 1.95 μg/mL of streptomycin, respectively 3.9 μg/mL MIC after the exposure of MRSA BAA-1720 to artonin E or less than 1.0 μg/mL MIC to Streptomycin. Post-antibacterial effects of artonin E and streptomycin in Staphylococcus aureus ATCC 25923 and MRSA BAA-1720.细菌生长抑制活性以毫米（mm）为单位表征，采用纸片扩散法（disc diffusion assay）进行检测。实验设置三次生物学重复，最终给出抑菌圈直径的平均值与标准差。鸡脚参素E（artonin E）对两株金黄色葡萄球菌（Staphylococcus aureus, S. aureus）的最小抑菌浓度（Minimum Inhibitory Concentration, MIC）与最小杀菌浓度（Minimum Bactericidal Concentration, MBC）水平相近；采用链霉素（streptomycin）所测得的MIC与MBC值亦与之相当。结果显示，金黄色葡萄球菌ATCC 25923分别暴露于3.9 μg/mL的鸡脚参素E、1.95 μg/mL的链霉素后均未生长；耐甲氧西林金黄色葡萄球菌（Methicillin-resistant Staphylococcus aureus, MRSA）BAA-1720在暴露于3.9 μg/mL的鸡脚参素E后未出现生长，或暴露于浓度低于1.0 μg/mL的链霉素时亦无细菌增殖。 鸡脚参素E与链霉素对金黄色葡萄球菌ATCC 25923及耐甲氧西林金黄色葡萄球菌BAA-1720的抗菌后效应。