Amplicon sequencing of 16S rRNA gene

Genomic DNA was extracted from granular biomass collected from an up-flow column reactor fed with ammonium, nitrite, phenol and thiocyanate. Partial sequences of the prokaryotic 16S rRNA gene was amplified using 515F and 806R primers, and the PCR amplicon was subjected to sequencing using the illumina MiSeq sequencer.本研究从以铵、亚硝酸盐、苯酚及硫氰酸盐为进料基质的上流式柱式反应器所采集的颗粒生物质中提取基因组DNA。采用515F与806R引物扩增原核生物16S核糖体RNA（16S rRNA）基因的部分序列，随后将聚合酶链式反应（PCR）扩增产物应用Illumina MiSeq测序仪完成测序。