Raw Data-Manuscript_The deubiquitinase OTUD1 negatively regulates inflammation and plays an immunoregulatory role in sepsis-induced lung injury

These additional files are uploaded as the "Raw Data of Original Materials", which we would like to share for review purpose only. Document List for all the Raw data: Raw data 1: Demographics and Clinical characterization of patients; Main clinical indexes and inflammatory biomarkers in sepsis patients. Raw data 2: The Detailed Transcriptomics profile of clinical sepsis patients; Public array-based profiling of sepsis patients from the GEO database of NCBI: GSE57065; GSE28750; GSE76293. Raw data 3： The Detailed Proteomics profile and primary bioinformatics analysis of OTUD1 KO mice. Raw data 4： The Detailed Transcriptomics profile and primary bioinformatics analysis of CLP-induced experimental sepsis in OTUD1 KO mice. Raw data 5： The original images for HE staining and Transmission electron microscopy；lung injury scores; lung W/D ratio；MPO, SOD and LDH activities；PaO2+PaO2/FiO2 ratio；survival rates. Raw data 6： The levels of inflammatory cytokines in the serum of mice (ELISA)：TNF-α, IL-6, IL-1β and HMGB1 levels. Raw data 7: The levels of inflammatory cytokines of LPS-stimulated BMDMs (qRT-PCR-1)：TNF‐α, IL-6, IL‐1β and HMGB1 expression levels; The levels of inflammatory cytokines of LPS-stimulated BMDMs by pretreatment with 5Z-7-ox (qRT-PCR-2)：TNF‐α, IL-6, IL‐1β and HMGB1 expression levels. Raw data 8 The activation of TAK1-MAPK/NF-κB pathways in LPS-stimulated BMDMs (Western Blot-1)：Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins; The activation of TAK1-MAPK/NF-κB pathways in LPS-stimulated BMDMs by pretreatment with 5Z-7-ox (Western Blot-2)：Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins; The activation of TAK1-MAPK/NF-κB pathways in lung tissues of mice (Western Blot-3)：Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins.

本次上传的补充文件归类为「原始素材原始数据（Raw Data of Original Materials）」，仅用于审核用途。所有原始数据的文档列表如下：
1. 原始数据1：患者人口统计学特征与临床表型分析；脓毒症患者主要临床指标及炎症生物标志物数据。
2. 原始数据2：临床脓毒症患者详细转录组学谱数据；来自NCBI基因表达综合数据库（Gene Expression Omnibus，GEO）的公开脓毒症患者芯片测序谱数据：GSE57065、GSE28750、GSE76293。
3. 原始数据3：OTUD1基因敲除小鼠的详细蛋白质组学谱及初步生物信息学分析数据。
4. 原始数据4：OTUD1基因敲除小鼠盲肠结扎穿刺（CLP）诱导的实验性脓毒症模型的详细转录组学谱及初步生物信息学分析数据。
5. 原始数据5：苏木精-伊红（HE）染色及透射电子显微镜原始图像；肺损伤评分、肺湿干重比、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）及乳酸脱氢酶（LDH）活性数据、动脉血氧分压（PaO2）及动脉血氧分压/吸入氧浓度（PaO2/FiO2）比值及小鼠生存率数据。
6. 原始数据6：小鼠血清炎症因子水平（酶联免疫吸附试验，ELISA）：肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）及高迁移率族蛋白B1（HMGB1）含量数据。
7. 原始数据7：脂多糖（LPS）刺激的骨髓来源巨噬细胞（Bone Marrow-derived Macrophages，BMDMs）炎症因子表达水平（实时定量聚合酶链反应1，qRT-PCR-1）：肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）及高迁移率族蛋白B1（HMGB1）的mRNA表达量数据；经5Z-7-ox预处理后脂多糖刺激的骨髓来源巨噬细胞炎症因子表达水平（实时定量聚合酶链反应2，qRT-PCR-2）：上述四种炎症因子的mRNA表达量数据。
8. 原始数据8：脂多糖（LPS）刺激的骨髓来源巨噬细胞中TAK1-MAPK/核因子κB（NF-κB）通路活化情况检测（蛋白质免疫印迹1，Western Blot-1）：TAK1、p38、JNK、ERK、p65及其各自磷酸化蛋白的表达水平数据；经5Z-7-ox预处理后脂多糖刺激的骨髓来源巨噬细胞中TAK1-MAPK/NF-κB通路活化情况检测（蛋白质免疫印迹2，Western Blot-2）：上述蛋白及其磷酸化形式的表达水平数据；小鼠肺组织中TAK1-MAPK/NF-κB通路活化情况检测（蛋白质免疫印迹3，Western Blot-3）：上述蛋白及其磷酸化形式的表达水平数据。