Distinct myeloid progenitor differentiation pathways uncovered through single cell RNA sequencing. Mus musculus

Haematopoietic stem cells can differentiate into all blood cell types. In this process, cells become progressively restricted to a single cell type. The order in which differentiating cells loose lineage potential, and the prospective isolation of cells with a defined potential remains a long-standing question. We performed gene expression analysis of haematopoietic cells from Gata1-EGFP reporter mice, leading to a model for hematopoiesis where the initial lineage decision consists of a seperation of erythroid/megakaryocyte/mast cell/eosinophil potential from lymphopoietic/monocyte/neutrophil potential Overall design: Find unbiased heterogeneity in the preGM hematopoietic progenitor population

造血干细胞（Haematopoietic stem cells）可分化为所有血细胞类型。在此过程中，细胞会逐步受限至单一细胞谱系。明确分化细胞丧失谱系潜能的时序，以及前瞻性分离具有确定谱系潜能的细胞，长期以来都是悬而未决的科学问题。本研究对源自Gata1-EGFP报告基因小鼠（Gata1-EGFP reporter mice）的造血细胞进行了基因表达分析，由此构建了造血模型：其中最初的谱系命运决定为红系/巨核系/肥大细胞/嗜酸性粒细胞谱系潜能与淋巴系/单核系/中性粒细胞谱系潜能的分离。研究设计概述：在preGM造血祖细胞群体中鉴定无偏倚的细胞异质性。