NanoPUZZLES ISA-TAB-Nano dataset: Zebrafish mortality and basic nanomaterial composition data extracted from Kovriznych et al. 2013 (doi:10.2478/intox-2013-0012)

This file is a ZIP archive which contains three different copies of an ISA-TAB-Nano dataset developed within the NanoPUZZLES EU project [http://www.nanopuzzles.eu]. The (meta)data in this dataset were primarily extracted from the following reference, with additional references consulted as indicated in the Investigation file: Zebrafish mortality and basic nanomaterial composition data extracted from Kovriznych et al. 2013 (doi:10.2478/intox-2013-0012)*****Before working with this dataset, you are strongly advised to read the following text - especially the "Disclaimers".*****ISA-TAB-Nano [1,2,3] has been proposed as a nanomaterial data exchange standard. As is explained in the README file contained within this dataset, as well as the "Investigation Description" field of the Investigation file regarding dataset specific deviations, the manner in which certain data and metadata were recorded within these datasets deviates from the expectations of the generic ISA-TAB-Nano specification. Marchese Robinson et al. [3], distributed within this dataset, discusses this in more detail. However, some additional new business rules, going beyond those described in Marchese Robinson et al. [3], may also have been applied to this dataset - as documented in the README file.This dataset was developed using Excel-based templates developed in the NanoPUZZLES project [4]. (N.B. The latest version of the templates, at the time of writing, was version 4 as opposed to version 3 which was described in Marchese Robinson et al. [3]. This latest version of the templates should be contained within the README file of this dataset.) Since these templates were iteratively updated, not all datasets may be perfectly consistent with the latest version - although efforts were made to minimise inconsistencies.The three copies of this dataset are as follows:<br>(a) 10.2478_FS_intox-2013-0012.zip: the original dataset prepared within Excel<br>(b) 10.2478_FS_intox-2013-0012-txt_opt-N.zip: a tab-delimited text version of this dataset prepared using version 2.0 of the cited Python program [5], with the -N flag selected (designed to minimise inconsistencies with the latest version of the NanoPUZZLES templates)<br>(c) 10.2478_FS_intox-2013-0012-txt_opt-a_opt-c_opt-N.zip: a tab-delimited text version of this dataset prepared using version 2.0 of the cited Python program [5], with the -N, -a (truncate ontology IDs) and -c (remove Investigation file comments) flags selected, as required for submission to the nanoDMS online database system [3,6]. ***At the time of writing, this dataset had not be submitted to the nanoDMS system due to technical issues.*** <br>Disclaimers:(1) this work has not undergone peer review<br>(2) no endorsement by third parties should be inferred<br>(3) *You are strongly advised to read the README file and the "Investigation Description" field of the Investigation file before working with this dataset. The latter field may document dataset specific caveats such as possible problems or uncertainties associated with curation from the original reference(s). Cited references:<br>[1] Thomas, D.G. et al. BMC Biotechnol. 2013, 13, 2. doi:10.1186/1472-6750-13-2<br>[2] https://wiki.nci.nih.gov/display/ICR/ISA-TAB-Nano (accessed 18th of December 2015)<br>[3] Marchese Robinson, R.L. et al. Beilstein J. Nanotechnol. 2015, 6, 1978–1999. doi:10.3762/bjnano.6.202<br>[4] http://www.myexperiment.org/files/1356.html (accessed 18th of December 2015)<br>[5] https://github.com/RichardLMR/xls2txtISA.NANO.archive (accessed 18th of December 2015)<br>[6] http://biocenitc-deq.urv.cat/nanodms (accessed 18th of December 2015)Funding:The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/ 2007-2013) under grant agreement no. 309837 (NanoPUZZLES project).<br>

本文件为ZIP压缩归档，包含欧盟NanoPUZZLES项目开发的三份不同副本的ISA-TAB-Nano（ISA-TAB-Nano）数据集，项目官网：http://www.nanopuzzles.eu。
本数据集的（元）数据主要源自以下参考文献，其余参考资料则按照研究记录（Investigation）文件中的标注进行参考：斑马鱼死亡率与基础纳米材料组成数据取自Kovriznych等人2013年的研究（DOI:10.2478/intox-2013-0012）。

**在使用本数据集前，强烈建议您阅读以下内容，尤其是「免责声明」部分。**

ISA-TAB-Nano[1,2,3]已被提出作为纳米材料数据交换标准。正如本数据集内置的README文件，以及研究记录文件中的「研究描述」字段所阐释的数据集特定偏差内容所示，本数据集内部分数据与元数据的记录方式，与通用ISA-TAB-Nano规范的预期存在差异。本数据集附带的Marchese Robinson等人[3]的文献对此进行了更为详尽的阐述。不过，本数据集可能还应用了部分超出Marchese Robinson等人[3]所述范围的新增业务规则，相关细节已在README文件中记录。

本数据集基于NanoPUZZLES项目[4]开发的Excel模板构建而成。（注：本文撰写时，该模板的最新版本为第4版，而非Marchese Robinson等人[3]中提及的第3版。本数据集的README文件中应包含该最新版模板。）由于该模板历经迭代更新，并非所有数据集都能与最新版模板完全契合，尽管已尽最大努力降低不一致性。

本数据集包含三个副本，详情如下：
(a) 10.2478_FS_intox-2013-0012.zip：基于Excel构建的原始数据集
(b) 10.2478_FS_intox-2013-0012-txt_opt-N.zip：采用所引用的Python程序[5]第2.0版生成的制表符分隔文本格式数据集，启用了-N参数（旨在降低与NanoPUZZLES最新版模板的不一致性）
(c) 10.2478_FS_intox-2013-0012-txt_opt-a_opt-c_opt-N.zip：采用所引用的Python程序[5]第2.0版生成的制表符分隔文本格式数据集，启用了-N、-a（截断本体标识符）和-c（移除研究记录文件注释）参数，符合提交至nanoDMS在线数据库系统[3,6]的要求。***本文撰写时，本数据集因技术问题尚未提交至nanoDMS系统。***

免责声明：
(1) 本作品尚未经过同行评审
(2) 不得推断本作品获得任何第三方背书
(3) **强烈建议您在使用本数据集前，阅读README文件以及研究记录文件中的「研究描述」字段。该字段可能会记录本数据集的特定注意事项，例如源自原始参考文献的整理工作可能存在的问题或不确定性。**

参考文献：
[1] Thomas, D.G. 等人. BMC Biotechnol. 2013, 13, 2. DOI:10.1186/1472-6750-13-2
[2] https://wiki.nci.nih.gov/display/ICR/ISA-TAB-Nano（2015年12月18日访问）
[3] Marchese Robinson, R.L. 等人. Beilstein J. Nanotechnol. 2015, 6, 1978–1999. DOI:10.3762/bjnano.6.202
[4] http://www.myexperiment.org/files/1356.html（2015年12月18日访问）
[5] https://github.com/RichardLMR/xls2txtISA.NANO.archive（2015年12月18日访问）
[6] http://biocenitc-deq.urv.cat/nanodms（2015年12月18日访问）

资助说明：
本研究成果的研发工作获得了欧盟第七框架计划（FP7/2007-2013）下编号为309837的资助协议支持（NanoPUZZLES项目）。