Gene expression profile of thalamus based on RNA-seq

The impact of neurons on the pathogenesis of central poststroke pain (CPSP) after thalamic hemorrhage is currently unknown. The aim of this study was to identify the key genes in neurons and investigate their roles in pain after thalamic hemorrhage based on RNA-sequencing (RNA-seq) data from model mice. The differentially expressed genes (DEGs) in neurons between the model and control groups were identified. A protein‒protein interaction (PPI) network of the DEGs was constructed. CytoNCA was utilized to analyze the connectivity of the nodes in the PPI network, and the top 5 genes were identified as key genes in the network. Afterward, an miRNA‒mRNA network and TF-mRNA network were established.

当前，神经元对丘脑出血后卒中后中枢痛（central poststroke pain, CPSP）发病机制的影响尚不明确。本研究旨在基于模型小鼠的RNA测序（RNA-sequencing, RNA-seq）数据，鉴定神经元中的关键基因并探究其在丘脑出血后疼痛中的作用。研究人员首先鉴定了模型组与对照组神经元间的差异表达基因（differentially expressed genes, DEGs），构建了该批差异表达基因的蛋白质-蛋白质相互作用（protein-protein interaction, PPI）网络。利用CytoNCA分析PPI网络中各节点的连接度，并筛选出排名前五的基因作为该网络的关键基因。随后构建了microRNA-信使RNA（miRNA-mRNA）调控网络与转录因子-信使RNA（TF-mRNA）调控网络。