Table_2_Hypoallergen Peanut Lines Identified Through Large-Scale Phenotyping of Global Diversity Panel: Providing Hope Toward Addressing One of the Major Global Food Safety Concerns.xlsx

Peanut allergy is one of the serious health concern and affects more than 1% of the world’s population mainly in Americas, Australia, and Europe. Peanut allergy is sometimes life-threatening and adversely affect the life quality of allergic individuals and their families. Consumption of hypoallergen peanuts is the best solution, however, not much effort has been made in this direction for identifying or developing hypoallergen peanut varieties. A highly diverse peanut germplasm panel was phenotyped using a recently developed monoclonal antibody-based ELISA protocol to quantify five major allergens. Results revealed a wide phenotypic variation for all the five allergens studied i.e., Ara h 1 (4–36,833 µg/g), Ara h 2 (41–77,041 µg/g), Ara h 3 (22–106,765 µg/g), Ara h 6 (829–103,892 µg/g), and Ara h 8 (0.01–70.12 µg/g). The hypoallergen peanut genotypes with low levels of allergen proteins for Ara h 1 (4 µg/g), Ara h 2 (41 µg/g), Ara h 3 (22 µg/g), Ara h 6 (829 µg/g), and Ara h 8 (0.01 µg/g) have paved the way for their use in breeding and genomics studies. In addition, these hypoallergen peanut genotypes are available for use in cultivation and industry, thus opened up new vistas for fighting against peanut allergy problem across the world.

花生过敏是一类严重的健康问题，影响全球超过1%的人口，主要集中在美洲、澳大利亚与欧洲地区。花生过敏有时会危及生命，且会对过敏个体及其家庭的生活质量造成负面影响。食用低变应原花生是当前最佳的解决方案，但目前针对鉴定或培育低变应原花生品种的相关研究投入仍较为有限。本研究采用新近开发的基于单克隆抗体的酶联免疫吸附测定（ELISA）方案，对一个高度多样化的花生种质资源群体开展表型分析以定量检测五种主要过敏原，结果显示所研究的五种过敏原均存在广泛的表型变异：Ara h 1（4–36,833 µg/g）、Ara h 2（41–77,041 µg/g）、Ara h 3（22–106,765 µg/g）、Ara h 6（829–103,892 µg/g）以及Ara h 8（0.01–70.12 µg/g）。其中过敏原蛋白含量较低的低变应原花生基因型（Ara h 1为4 µg/g、Ara h 2为41 µg/g、Ara h 3为22 µg/g、Ara h 6为829 µg/g、Ara h 8为0.01 µg/g），为其在育种与基因组学研究中的应用铺平了道路。此外，此类低变应原花生基因型可应用于种植与产业环节，从而为全球范围内对抗花生过敏问题开辟了全新的前景。