Additional file 1 of Long-read sequencing of the zebrafish genome reorganizes genomic architecture

Additional file 1: Figure S1. Read length distribution and sequenced bases generated by each group across all libraries used in assembly generation. Figure S2. Tukey box and whiskers plot of average depth at the telomeric regions of all chromosomes in the zebrafish genome. Figure S3. Association plot of Chr 4 in ZF1 and GRCz11 assemblies illustrating many small sequence differences between the two builds. Figure S4. BUSCO analysis of GRCz11 reference assembly and ZF1 assembly using vertebrate-specific single-copy orthologs. Table S1. Chromosomal location of GRCz11 unlocalized scaffolds bearing > 99% coverage in GRCz11. Table S2. Deletions mapped to insertions in ZF1 assembly. Table S3. Primers used for RT-qPCR.

附加文件1：图S1。各测序组在用于基因组组装构建的全部测序文库中产生的读长分布与测序总碱基数。
图S2。斑马鱼基因组所有染色体端粒区域平均测序深度的图基箱线图（Tukey box and whiskers plot）。
图S3。ZF1与GRCz11两个组装版本中4号染色体（Chr 4）的关联图谱，展示了二者间存在大量微小序列差异。
图S4。基于脊椎动物特异性单拷贝同源基因的GRCz11参考基因组组装与ZF1基因组组装的BUSCO分析。
表S1。GRCz11参考基因组中覆盖度≥99%的未定位测序支架（unlocalized scaffolds）的染色体定位信息。
表S2。ZF1组装版本中比对至插入位点的缺失序列信息。
表S3。实时定量反转录PCR（RT-qPCR）所用引物。