Gene expression analysis of molecules with known function in HLA class II antigen processing and presentation in hematopoietic and (cytokine pre-treated) non-hematopoietic cells.

Gene expression analysis of molecules with known function in HLA class II antigen processing and presentation. Various hematopoietic cell types and (cytokine pre-treated) non-hematopoietic cells that are targeted in Graft-versus-Leukemia reactivity and Graft-versus-Host Disease were collected. Expression was compared between the different hematopoietic and non-hematopoietic cell types for the Invariant chain, HLA-DMA, HLA-DMB, HLA-DOA and HLA-DOB genes. The data show that the Invariant chain, HLA-DMA, HLA-DMB and HLA-DOA genes are expressed in all or the majority of cell types with HLA class II surface expression, whereas expression of the HLA-DOB gene is restricted to professional antigen presenting B-cells and mature dendritic cells. Total RNA was isolated from various hematopoietic cell types isolated (and cultured) from (G-CSF mobilized) peripheral blood from five different individuals and from (IFN-g pre-treated) fibroblasts cultured from skin biopsies from four different patients transplanted with allogeneic hematopoietic stem cells.

本数据集针对参与HLA II类抗原加工与呈递（HLA class II antigen processing and presentation）且功能已明确的分子开展基因表达分析。收集了移植物抗白血病反应（Graft-versus-Leukemia reactivity）与移植物抗宿主病（Graft-versus-Host Disease）所靶向的多种造血细胞类型，以及经细胞因子预处理的非造血细胞。针对恒常链（Invariant chain）、HLA-DMA、HLA-DMB、HLA-DOA及HLA-DOB基因，比较不同造血与非造血细胞类型的基因表达水平。数据分析结果显示，恒常链、HLA-DMA、HLA-DMB及HLA-DOA基因在所有或多数表达HLA II类表面抗原的细胞类型中均有表达，而HLA-DOB基因的表达仅局限于专职抗原呈递B细胞与成熟树突状细胞。总RNA提取自5名不同个体经粒细胞集落刺激因子（G-CSF）动员的外周血中分离并培养的多种造血细胞类型，以及4名接受异基因造血干细胞移植（allogeneic hematopoietic stem cells）患者的皮肤活检组织分离、经干扰素γ（IFN-γ）预处理的成纤维细胞培养物。