Microarray profiling analysis of microRNAs expression in diabetes samples

At present, there is no effective treatment for diabetic wounds, and the cost of treatment is high. MicroRNAs (miRNAs) plays an important role in the process of diabetic wound healing. By regulating the expression of target genes, it regulates growth factors, cytokines and signal pathways, thereby affecting various stages of ulcer healing such as hemostasis, anti-inflammatory, proliferation and remodeling. In this study, differential expression of miRNAs in diabetic wound was screened. MiR-206 was selected as the research object to detect the effect of miR-206 on the proliferation of fibroblasts and vascular endothelial cell by regulating HIF-1?. Finally, in vivo studies showed that miR-206 antagomir could promote the expression of HIF-1?, CD34 and VEGF, and further promote wound healing in diabetic rats. Three normal skin samples and three diabetes samples were collected in the present study.

目前，糖尿病创面尚无有效治疗手段，且治疗成本高昂。微小RNA（MicroRNAs, miRNAs）在糖尿病创面愈合过程中发挥关键调控作用。其通过调控靶基因的表达，调节生长因子、细胞因子及信号通路，进而影响止血、抗炎、增殖与重塑等溃疡愈合的各个阶段。本研究筛选出糖尿病创面中差异表达的miRNAs，选取miR-206作为研究对象，探究其通过调控缺氧诱导因子-1α（HIF-1α）对成纤维细胞与血管内皮细胞增殖的影响。最终，体内实验结果证实，miR-206拮抗寡核苷酸（miR-206 antagomir）可上调缺氧诱导因子-1α、CD34抗原及血管内皮生长因子（VEGF）的表达，进一步促进糖尿病大鼠的创面愈合。本研究共收集3份正常皮肤样本与3份糖尿病创面样本。