Analysis of the taxonomic profile of the intestinal microbiota in patients with alcoholic liver cirrhosis and acute-on-chronic liver failure (ACLF) in western Mexico (dataset 1/2)

ACLF is a severe stage of liver cirrhosis, characterized by multiple organ failure, systemic inflammation, and high short-term mortality. The intestinal microbiota (IM) influences its pathophysiology; however, as of the time of main publication, there were no studies in Latin American populations. Therefore, we analyzed IM and its relationships with several clinical outcomes. The IM was characterized using excreted fecal samples, from which DNA was extracted using Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research); then, sequencing was achieved using using standard 16S-rRNA V3-V4 Illumina protocol (2x300 bp). This record comprises the first data set (1/2).慢加急性肝衰竭（ACLF）是肝硬化的危重阶段，以多器官功能衰竭、全身炎症反应及较高的短期死亡率为核心特征。肠道菌群（intestinal microbiota, IM）可影响其病理生理过程；但截至主刊发表之际，针对拉丁美洲人群的相关研究仍属空白。为此，本研究对肠道菌群及其与多项临床结局的关联展开分析。研究采集受试者的粪便标本以表征肠道菌群，使用Quick-DNA粪便/土壤微生物微量提取试剂盒（Zymo Research）提取粪便DNA；随后采用标准16S-rRNA V3-V4区Illumina测序方案（2×300 bp）完成测序。本记录包含首个数据集（1/2）。