Co-option of neutrophil fates by tissue environments

Classically considered short-lived, purely defensive leukocytes, neutrophils are unique in their fast and moldable response to stimulation. This plastic behavior may underlie variable and even antagonistic functions during inflammation or cancer, yet the full spectrum of neutrophil properties as they enter healthy tissues remains unexplored. Using a new model to track neutrophil fates, we found short but variable lifetimes across multiple tissues. Through analysis of the receptor, transcriptional and chromatin accessibility landscapes, we identify varying neutrophil states and assign non-canonical functions, including vascular repair and hematopoietic homeostasis. scRNASeq data were generated from different tissues, namely Bone Marrow (BM), peripheral blood (PB), Lung and Spleen in biological quadruplicate from healthy WT mice using the 10X Chromium platform