Mesenchymal Stem Cells Generated from Human Urine-derived iPSCs Exhibit Low Immunogenicity and Im-munomodulatory Profile

We conducted transcriptome analysis to determine the differences between umbilical cord-derived mesenchymal stem cells (named UCMSC) and iMSCs generated from human urine-derived iPSC (named B6MSC) at the transcriptomic level. The expression of genes related to aging, rejuvenation, proliferation or immune regulatory factors in iMSCs was different significantly from that of UCMSCs. Overall design: Comparative gene expression profiling analysis of RNA-seq data for UCMSCs and iMSCs generated from human urine-drived iPSC.

本研究开展转录组分析，旨在从转录组水平上明确脐带源间充质干细胞（umbilical cord-derived mesenchymal stem cells，简称UCMSC）与人类尿液来源诱导多能干细胞（induced pluripotent stem cell，iPSC）诱导生成的诱导性间充质干细胞（induced mesenchymal stem cells，iMSCs，命名为B6MSC）之间的表达差异。iMSCs中与衰老、细胞复壮、细胞增殖或免疫调节因子相关的基因表达水平，与UCMSCs存在显著差异。整体实验设计：针对UCMSCs及人类尿液来源诱导多能干细胞诱导生成的iMSCs的RNA测序（RNA-seq）数据开展比较基因表达谱分析。