gene expression profile after GGPPS deletion in sertoli cell

GGPPS was the key enzyme of mevalonate metabolic pathway which was used to synthesize the geranylgeranyl pyrophosphate (GGPP). When we deletion the GGPPS in the sertoli cell and the germ cell loss were found . We used microarrays to detail the global programme of gene expression after GGPPS deletion in sertoli cell and identified 1623 gene expression level change more than 2 times. Primary sertoli cells from 3 days old of control and KO mice for RNA extraction and hybridization on Affymetrix microarrays, every group primare sertoli cell were from more than 30 mice.

牻牛儿基牻牛儿基焦磷酸合酶（GGPPS）是甲羟戊酸代谢通路的关键酶，可催化合成牻牛儿基牻牛儿基焦磷酸（geranylgeranyl pyrophosphate，GGPP）。在支持细胞（Sertoli cell）中敲除GGPPS后，可观察到生殖细胞丢失现象。本研究通过微阵列（microarray）技术解析了支持细胞中GGPPS敲除后的全基因表达谱，共鉴定出1623个表达量变化幅度超过2倍的差异基因。本研究使用3日龄野生型与敲除（KO）小鼠的原代支持细胞进行RNA提取，并在Affymetrix微阵列平台完成杂交实验；每组原代支持细胞均取自超过30只小鼠。