Data Sheet 1_Changes in the Subdoligranulum genus in patients with autoimmune disease: a systematic review and meta-analysis.zip

BackgroundAutoimmune diseases have different pathogenic mechanisms but share underlying patterns of gut microbiome perturbation and intestinal barrier dysfunction. Recent evidence suggests that an arthritogenic strain of Subdoligranulum causes a local inflammatory response in the gut. Therefore, the aim of this review was to systematically summarize the relationships between Subdoligranulum and multiple autoimmune diseases.
ObjectiveTo evaluate the changes of Subdoligranulum in different autoimmune diseases.
MethodsFour databases, including PubMed, Cochrane, Web of Science, and Embase, were searched up to June 17, 2025, to identify studies that detected Subdoligranulum in autoimmune diseases. A meta-analysis was conducted to compare the differences in Subdoligranulum between healthy people and patients with autoimmune diseases, and the changes in these bacteria under different treatments were compared for similar diseases. The relationships between Subdoligranulum and inflammation-related biomarkers were also analyzed.
Study selectionWe included articles that addressed both autoimmune diseases without intervention and the detection of Subdoligranulum in feces, and we presented a description of changes in bacteria in patients and healthy controls.
Quality assessmentWe used the Newcastle–Ottawa Scale (NOS) to independently assess the methodological quality of the case–control studies. The Journal of Biomedical Informatics (JBI) critical appraisal checklists were utilized to assess the quality and risk of bias in cross-sectional studies.
ResultsTwelve studies were included. These studies were conducted in four different countries and included a total of 1,792 participants (patients with autoimmune disease and healthy controls). Our meta-analysis results indicate that, compared with healthy controls, most patients with autoimmune diseases included in the study had lower levels of Subdoligranulum (p = 0.027). In addition, it was found that bacteria were associated with several inflammation-related biomarkers. For example, bacterial levels were positively correlated with C-reactive protein (CRP), lipopolysaccharide (LPS)-binding protein (LBP), and Treg cells. However, the levels were negatively correlated with IL-8. These relationships may underlie both the occurrence and development of autoimmune diseases.
ConclusionThe abundance of Subdoligranulum in patients with organ-specific autoimmune diseases was decreased, whereas no consistent findings were observed for systemic autoimmune diseases.
Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/view/CRD42024543767, identifier CRD42024543767.

背景 自身免疫性疾病（Autoimmune diseases）具有各异的致病机制，但均存在肠道菌群紊乱与肠屏障功能障碍的共同特征。近期研究表明，一种致关节炎性的戴沃斯菌属（Subdoligranulum）菌株可引发肠道局部炎症反应。因此本综述旨在系统总结戴沃斯菌属与多种自身免疫性疾病之间的关联。

目的 评估不同自身免疫性疾病中戴沃斯菌属的丰度变化。

方法 检索截至2025年6月17日的PubMed、Cochrane、Web of Science及Embase四大数据库，筛选出在自身免疫性疾病中检测戴沃斯菌属的相关研究。本研究采用荟萃分析，对比健康人群与自身免疫性疾病患者体内戴沃斯菌属的丰度差异，并针对同类疾病，比较不同干预措施下该菌属的丰度变化；同时分析戴沃斯菌属与炎症相关生物标志物的关联。

研究筛选 纳入同时涵盖未接受干预的自身免疫性疾病患者、粪便样本中戴沃斯菌属检测结果的文献，并呈现患者与健康对照的菌群变化情况。

质量评估 采用纽卡斯尔-渥太华量表（Newcastle–Ottawa Scale, NOS）独立评估病例对照研究的方法学质量；采用《生物医学信息学杂志》（Journal of Biomedical Informatics, JBI）关键评价清单评估横断面研究的质量与偏倚风险。

结果 最终纳入12项研究，涉及4个国家，共1792名受试者（自身免疫性疾病患者与健康对照）。荟萃分析结果显示，相较于健康对照，本研究纳入的多数自身免疫性疾病患者体内戴沃斯菌属的丰度显著降低（p=0.027）。此外，研究发现该菌属与多种炎症相关生物标志物存在关联：其丰度与C反应蛋白（C-reactive protein, CRP）、脂多糖结合蛋白（lipopolysaccharide-binding protein, LBP）及调节性T细胞（Treg cells）呈正相关，而与白细胞介素-8（IL-8）呈负相关。上述关联或可为自身免疫性疾病的发生与发展提供潜在机制。

结论 器官特异性自身免疫性疾病患者体内戴沃斯菌属的丰度降低，而全身性自身免疫性疾病的相关研究结果尚未达成一致。

系统综述注册 注册号为CRD42024543767，注册链接：https://www.crd.york.ac.uk/PROSPERO/view/CRD42024543767。