Nicotiana benthamiana inoculated with grapevine fanleaf virus

A symptom determinant lies within residue 802 of 1EPol of RNA1 in grapevine fanleaf virus. Two wild strains (GHu and F13) of GFLV and two mutant strains (GHu 1EPol K802G and F13 1EPol G802K) were mechanically introduced into ~5 week old N. benth. Tissue from these plants was collected at 4, 7, and 12 days post inoculation in five biological replicates with pooling of 3 individuals per biorep. The goal of this study was to uncover host immune response with respect to viral treatment and other modified host metabolism components. This data set has a complementary protein analysis available at ProteomeExchange.

葡萄扇叶病毒（Grapevine fanleaf virus, GFLV）RNA1编码的1EPol蛋白第802位残基为其症状决定因子。本研究选取GFLV的2个野生株系（GHu与F13）及2个突变株系（GHu 1EPol K802G与F13 1EPol G802K），通过机械接种方式将其接种至约5周龄的本氏烟草（Nicotiana benthamiana，缩写为N. benth.）植株中。分别于接种后4、7、12天采集植株组织，设置5个生物学重复，每个生物学重复混合3株个体的组织样本。本研究旨在解析病毒处理相关的宿主免疫应答，以及宿主代谢组分的改变情况。本数据集配有可在ProteomeExchange数据库获取的配套蛋白质组学分析数据。