Effects of StcE treatment on in vitro brain endothelial cell line bEnd.3. Effects of StcE treatment on in vitro brain endothelial cell line bEnd.3

Mucin-domain glycoproteins populate the brain endothelial cell surface, but their roles as a class of glycoconjugates are largely unknown. Here, we perform bulk RNA-sequencing of bEnd.3 cells treated with StcE, which enzymatically degrades cell surface mucins, in order to understand the effects of mucin removal on brain endothelial cell function. Overall design: Bulk RNA-sequencing of bEnd.3 cells treated with StcE or saline in growth media

粘蛋白结构域糖蛋白（Mucin-domain glycoproteins）富集于脑内皮细胞表面，但作为一类糖缀合物，其整体生物学功能仍鲜为人知。本研究对经StcE处理的bEnd.3细胞进行批量RNA测序（bulk RNA-sequencing），其中StcE可酶促降解细胞表面粘蛋白，旨在明确去除粘蛋白对脑内皮细胞功能的影响。整体实验设计：在生长培养基中，分别用StcE与生理盐水处理bEnd.3细胞，随后对其开展批量RNA测序。