Transcriptome analysis of the response of domesticated emmer wheat (Triticum turgidum subsp. dicoccum) to single vs. mixed infections with Zymosptoria tritici and Puccinia graminis

Tetraploid emmer Triticum turgidum subsp. dicoccum is one of the first wheat species to be domesticated. Emmer shares many pathogen species with modern durum wheat (Triticum turgidum subsp. durum) and hexaploid bread wheat (Triticum aestivum L.). Domesticated emmer is considered a highly valuable source of potentially novel disease resistance genes that can be introduced into modern wheat varieties, however, large gaps remain in our understanding of the basic immune responses of emmer to the most common wheat pathogens. In this work we describe the transcriptional response of emmer to single and mixed pathogen species infections with the ascomycete Zymoseptoria tritici, and the basidiomycete Puccinia graminis. In doing so, we here uncover unique patterns in the response of emmer to a more complex pathogen population with mixed species infections. Host Treatment_1 Treatment_2 Gene expression data Paired-end Library_1 Paired-end Library_2 Triticum turgidum subsp dicoccum Mock Mock mmc_ztsr_svevo_501_quant.sf P29206_501_S1_L003_R1_001.fastq.gz P29206_501_S1_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Mock Mock mmc_ztsr_svevo_502_quant.sf P29206_502_S2_L003_R1_001.fastq.gz P29206_502_S2_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Mock Mock mmc_ztsr_svevo_503_quant.sf P29206_503_S3_L003_R1_001.fastq.gz P29206_503_S3_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Zymoseptoria tritici Mock mzc_ztsr_svevo_504_quant.sf P29206_504_S4_L003_R1_001.fastq.gz P29206_504_S4_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Zymoseptoria tritici Mock mzc_ztsr_svevo_505_quant.sf P29206_505_S5_L003_R1_001.fastq.gz P29206_505_S5_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Zymoseptoria tritici Mock mzc_ztsr_svevo_506_quant.sf P29206_506_S6_L003_R1_001.fastq.gz P29206_506_S6_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Mock Puccinia graminis mmsr_ztsr_svevo_507_quant.sf P29206_507_S7_L003_R1_001.fastq.gz P29206_507_S7_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Mock Puccinia graminis mmsr_ztsr_svevo_508_quant.sf P29206_508_S8_L003_R1_001.fastq.gz P29206_508_S8_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Mock Puccinia graminis mmsr_ztsr_svevo_509_quant.sf P29206_509_S9_L003_R1_001.fastq.gz P29206_509_S9_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Zymoseptoria tritici Puccinia graminis mzsr_ztsr_svevo_510_quant.sf P29206_510_S10_L003_R1_001.fastq.gz P29206_510_S10_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Zymoseptoria tritici Puccinia graminis mzsr_ztsr_svevo_511_quant.sf P29206_511_S11_L003_R1_001.fastq.gz P29206_511_S11_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum Zymoseptoria tritici Puccinia graminis mzsr_ztsr_svevo_512_quant.sf P29206_512_S12_L003_R1_001.fastq.gz P29206_512_S12_L003_R2_001.fastq.gz四倍体二粒小麦（*Triticum turgidum* subsp. *dicoccum*）是最早被驯化的小麦物种之一。该类二粒小麦与现代硬粒小麦（*Triticum turgidum* subsp. *durum*）以及六倍体普通小麦（*Triticum aestivum* L.）共享诸多病原菌类群。驯化二粒小麦被视为极具价值的新型抗病基因资源，可将其导入现代小麦品种中，但目前学界对二粒小麦针对最常见小麦病原菌的基础免疫应答机制仍存在大量认知空白。本研究阐述了二粒小麦在单独接种及混合接种子囊菌小麦叶枯病菌（*Zymoseptoria tritici*）和担子菌小麦秆锈病菌（*Puccinia graminis*）后的转录应答特征。借此，本研究揭示了二粒小麦在面对包含多物种的复杂病原菌种群时的独特应答模式。 宿主 处理1 处理2 基因表达数据 双端文库1 双端文库2 Triticum turgidum subsp dicoccum 空白对照 空白对照 mmc_ztsr_svevo_501_quant.sf P29206_501_S1_L003_R1_001.fastq.gz P29206_501_S1_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 空白对照 空白对照 mmc_ztsr_svevo_502_quant.sf P29206_502_S2_L003_R1_001.fastq.gz P29206_502_S2_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 空白对照 空白对照 mmc_ztsr_svevo_503_quant.sf P29206_503_S3_L003_R1_001.fastq.gz P29206_503_S3_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 小麦叶枯病菌（Zymoseptoria tritici） 空白对照 mzc_ztsr_svevo_504_quant.sf P29206_504_S4_L003_R1_001.fastq.gz P29206_504_S4_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 小麦叶枯病菌（Zymoseptoria tritici） 空白对照 mzc_ztsr_svevo_505_quant.sf P29206_505_S5_L003_R1_001.fastq.gz P29206_505_S5_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 小麦叶枯病菌（Zymoseptoria tritici） 空白对照 mzc_ztsr_svevo_506_quant.sf P29206_506_S6_L003_R1_001.fastq.gz P29206_506_S6_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 空白对照 小麦秆锈病菌（Puccinia graminis） mmsr_ztsr_svevo_507_quant.sf P29206_507_S7_L003_R1_001.fastq.gz P29206_507_S7_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 空白对照 小麦秆锈病菌（Puccinia graminis） mmsr_ztsr_svevo_508_quant.sf P29206_508_S8_L003_R1_001.fastq.gz P29206_508_S8_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 空白对照 小麦秆锈病菌（Puccinia graminis） mmsr_ztsr_svevo_509_quant.sf P29206_509_S9_L003_R1_001.fastq.gz P29206_509_S9_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 小麦叶枯病菌（Zymoseptoria tritici） 小麦秆锈病菌（Puccinia graminis） mzsr_ztsr_svevo_510_quant.sf P29206_510_S10_L003_R1_001.fastq.gz P29206_510_S10_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 小麦叶枯病菌（Zymoseptoria tritici） 小麦秆锈病菌（Puccinia graminis） mzsr_ztsr_svevo_511_quant.sf P29206_511_S11_L003_R1_001.fastq.gz P29206_511_S11_L003_R2_001.fastq.gz Triticum turgidum subsp dicoccum 小麦叶枯病菌（Zymoseptoria tritici） 小麦秆锈病菌（Puccinia graminis） mzsr_ztsr_svevo_512_quant.sf P29206_512_S12_L003_R1_001.fastq.gz P29206_512_S12_L003_R2_001.fastq.gz