Summary of the results of cell fusion and hybridoma selection.
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aWells with antibodies that recognized 1 µg/mL of DBAP-BSA conjugate by inELISA (absorbance >1.0).bWells with antibodies that recognized free DBP (inhibition >50% by 5 µg/mL DBP). IcELISA was carried out with 1 µg/mL DBAP-BSA, and culture supernatants giving absorbances out of range were diluted until absorbance about 1.0.cHybridomas secreting antibodies with the lowest IC50 for DBP were stabilized and cloned.
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2015-12-02



