Summary of the results of cell fusion and hybridoma selection.

aWells with antibodies that recognized 1 µg/mL of DBAP-BSA conjugate by inELISA (absorbance >1.0).bWells with antibodies that recognized free DBP (inhibition >50% by 5 µg/mL DBP). IcELISA was carried out with 1 µg/mL DBAP-BSA, and culture supernatants giving absorbances out of range were diluted until absorbance about 1.0.cHybridomas secreting antibodies with the lowest IC50 for DBP were stabilized and cloned.a. 经间接酶联免疫吸附试验（inELISA）检测，可识别1微克/毫升DBAP-牛血清白蛋白（BSA）结合物且吸光度＞1.0的反应孔。b. 可识别游离DBP，且在5微克/毫升DBP存在时抑制率＞50%的反应孔。c. 采用1微克/毫升DBAP-BSA进行酶联免疫吸附试验，对于吸光度超出检测范围的细胞培养上清液，需进行稀释直至吸光度约为1.0。d. 将分泌对DBP具有最低半数抑制浓度（IC50）抗体的杂交瘤细胞进行稳定化处理并克隆。