Vascular endothelial growth factor, endostatin levels and clinical features among patients with ulcerative colitis and irritable bowel syndrome and among healthy controls: a cross-sectional analytical study

ABSTRACT BACKGROUND: Increased angiogenetic activity in inflammatory bowel disease (IBD) has been shown in previous studies. The aim of this study was to evaluate the relationship of serum vascular endothelial growth factor (VEGF) and endostatin levels with clinical features and mucosal expression in patients with ulcerative colitis (UC). DESIGN AND SETTING: Cross-sectional analytical study conducted in a tertiary-level public hospital. METHODS: Serum VEGF and endostatin levels were determined in 82 individuals: 39 with UC, 28 with irritable bowel syndrome (IBS) and 15 healthy controls (HCs), using enzyme-linked immunosorbent assays (ELISA). VEGF and endostatin expressions were studied using immunohistochemistry (IHC). RESULTS: Mean serum VEGF and endostatin levels were significantly higher in patients with UC than in patients with IBS and in HCs (511.9 ± 377.5 pg/ml, 305.0 ± 121.42 pg/ml and 36.1 ± 40.6 pg/ml; P = 0.001 for VEGF; and 155.50 ± 59.8 ng/ml, 116.9 ± 23.8 ng/ml and 102.2 ± 22.4 ng/ml; P

摘要 背景：既往研究已证实，炎症性肠病（inflammatory bowel disease, IBD）存在血管生成活性增强的现象。本研究旨在探讨溃疡性结肠炎（ulcerative colitis, UC）患者血清血管内皮生长因子（vascular endothelial growth factor, VEGF）及内皮抑素水平与临床特征及黏膜表达的相关性。
设计与研究场景：本研究为在三级公立医院开展的横断面分析研究。
方法：本研究纳入82名受试者，采用酶联免疫吸附试验（enzyme-linked immunosorbent assays, ELISA）检测其血清VEGF及内皮抑素水平，其中UC患者39例、肠易激综合征（irritable bowel syndrome, IBS）患者28例、健康对照（healthy controls, HCs）15例；采用免疫组织化学（immunohistochemistry, IHC）法检测VEGF及内皮抑素的黏膜表达情况。
结果：UC患者的血清VEGF及内皮抑素平均水平均显著高于IBS患者与健康对照者（VEGF分别为511.9 ± 377.5 pg/ml、305.0 ± 121.42 pg/ml及36.1 ± 40.6 pg/ml，VEGF组间比较P = 0.001；内皮抑素分别为155.50 ± 59.8 ng/ml、116.9 ± 23.8 ng/ml及102.2 ± 22.4 ng/ml；P