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Additional file 1: of Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content

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DataCite Commons2024-02-14 更新2024-07-27 收录
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Table S1. The result of ANOVA analysis for germination index and germination rate. Table S2. The peptide matches of DEPs. Table S3. The DEPs identified during germination in seeds with high and low oil content in B. napus. Table S4. Corresponding Arabidopsis homologous genes and STRING abbreviations of DEPs. Bolded font represents the genes that annotated as seed germination in NCBI database. Italic font represents the genes that annotated as seed specific in NCBI database. Table S5. Expression patterns of DEPs in germinating seeds with high and low oil content. H and L indicates that protein expression patterns of high and low oil-containing seeds, and the number indicates the hours after imbibition. Table S6. All of the QTLs for GP and GI detected in the present research. Table S7. The candidate genes identified by proteomic analysis. Table S8. The DEPs located within the QTLs for GP and GI. Black bold represent the gene that also founded in Table S7. Table S9. B. napus cultivars used in this research. Table S10. The setting of fluorescent dye labeling for protein samples. Table S11. The primers used in the present research. (XLSX 272 kb)

表S1 萌发指数与发芽率的方差分析(Analysis of Variance,ANOVA)结果。表S2 差异表达蛋白(Differentially Expressed Proteins,DEPs)的肽段匹配结果。表S3 甘蓝型油菜(Brassica napus,B. napus)高、低含油量种子萌发过程中鉴定得到的差异表达蛋白。表S4 差异表达蛋白对应的拟南芥同源基因及STRING数据库缩写。加粗字体代表在NCBI数据库中注释为种子萌发相关的基因,斜体字体代表在NCBI数据库中注释为种子特异性相关的基因。表S5 高、低含油量萌发种子中差异表达蛋白的表达模式。其中H与L分别代表高含油量与低含油量种子的蛋白表达模式,数字代表吸胀后的培养小时数。表S6 本研究中检测到的所有与发芽势(Germination Potential,GP)和萌发指数(Germination Index,GI)相关的数量性状位点(Quantitative Trait Locus,QTL)。表S7 蛋白质组学分析鉴定得到的候选基因。表S8 位于发芽势与萌发指数数量性状位点内的差异表达蛋白。黑色加粗字体代表同时出现在表S7中的基因。表S9 本研究使用的甘蓝型油菜品种。表S10 蛋白质样本的荧光染料标记设置方案。表S11 本研究使用的引物序列。(XLSX格式,272 KB)
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figshare
创建时间:
2019-01-12
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