Innate triggering and antiviral effector functions of activin A. Innate triggering and antiviral effector functions of activin A

Transcriptomic analyses delineated strikingly congruent patterns of gene regulation in hepatocytes stimulated with recombinant activin A and IFNα in vitro. Activin A mRNA, encoded by the INHBA gene, is induced upon activation of RIG-I, MDA5 and TLR7/8 viral nucleic acid sensors in vitro, across multiple cell lines and also in human peripheral blood mononuclear cells Overall design: we first used microarray analysis and RNA sequencing to analyse the effects of activin A upon hepatocytes at the transcriptional level and examine its intersection with the type I IFN axis

转录组学分析（Transcriptomic analysis）清晰揭示：体外条件下经重组激活素A（activin A）与干扰素α（IFNα）刺激的肝细胞，其基因调控模式呈现高度一致的特征。由INHBA基因编码的激活素A mRNA，可在体外多种细胞系及人外周血单个核细胞中，因RIG-I、MDA5与TLR7/8等病毒核酸传感器的激活而上调表达。实验整体设计：本研究首先通过微阵列分析（microarray analysis）与RNA测序（RNA sequencing）技术，在转录组水平分析激活素A对肝细胞的调控作用，并探究其与I型干扰素通路（type I IFN axis）的交叉关联。