The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.. The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.

The conserved miR-183/96/182 cluster (miR-183C) regulates both corneal sensory innervation and corneal resident immune cells (CRICs). This study is to uncover its role in CRICs and in shaping the corneal cellular landscape at a single-cell (sc) level. Overall design: Corneas of naïve, young adult [2 and 6 months old (mo)], female miR-183C knockout (KO) mice and wild-type (WT) littermates were harvested and dissociated into single cells. Dead cells were removed using a Dead Cell Removal kit. CD45 + CRICs were enriched by Magnetic Activated Cell Sorting (MACS). scRNA libraries were constructed and sequenced followed by comprehensive bioinformatic analyses

高度保守的miR-183/96/182簇（miR-183/96/182 cluster, miR-183C）可同时调控角膜感觉神经支配与角膜驻留免疫细胞（corneal resident immune cells, CRICs）。本研究旨在阐明其在CRICs中的调控功能，并于单细胞（single-cell, sc）水平解析角膜细胞图谱的塑造机制。实验整体设计：选取未经过处理的年轻成年雌性小鼠，包括2月龄与6月龄的miR-183C敲除（knockout, KO）小鼠及其野生型同窝对照小鼠，获取其角膜组织并解离为单细胞悬液；通过死细胞去除试剂盒去除死细胞，采用磁激活细胞分选（Magnetic Activated Cell Sorting, MACS）富集CD45阳性的CRICs；构建单细胞RNA测序文库并进行测序，随后开展全面的生物信息学分析。