Staphylococcus aureus transposon mutant libraries in wildtype and dMprF HG003

These libraries were used to determine genes that become essential in S. aureus in the absence of the lipid-modifying enzyme MprF. Genes in which transposon reads are significantly depleted in the dMprF library relative to the wildtype library don't tolerate transposon insertions when MprF is deleted and are therefore required for growth in dMprF. Knowledge of these genes can then be leveraged in chemical and genetic screens and selections.

本研究使用的这批文库，用于鉴定在缺失脂质修饰酶MprF的条件下，金黄色葡萄球菌（Staphylococcus aureus, S. aureus）体内成为必需的基因。相较于野生型文库，dMprF文库中转座子读段显著富集不足的基因，在MprF缺失的菌株中无法耐受转座子插入，因此是该菌株生长所必需的基因。上述基因的相关信息可应用于化学筛选、遗传筛选及遗传选择实验中。