Stenotrophomonas maltophilia FLR19 response to pH. Stenotrophomonas maltophilia FLR19 response to pH

We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes. Overall design: Bacterial transcriptional response to acidic, neutral and basic pH

本研究旨在明确一株源自囊性纤维化患者的嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia)分离株如何响应相关pH梯度（pH 5、7和9）：实验中将该细菌培养于磷酸盐缓冲培养基中，并开展RNA测序（RNAseq）实验。本研究数据表明，酸性环境对FLR19菌株具有胁迫作用，该菌株会通过上调应激响应与抗生素抗性基因的表达来应对该胁迫。实验整体设计：细菌在酸性、中性及碱性pH条件下的转录响应。