GSE47314: JHDM1_Q2634.OR Head Nuclei.Solexa

Summary: modENCODE_submission_5575 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Mixed Adult; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); tissue (organism part) ; Antibody JHDM1 Q2634 (target is JHDM1); Developmental Stage Mixed Adult

摘要：modENCODE_submission_5575
本提交数据源自Gary Karpen主导的modENCODE项目。如需查阅完整modENCODE项目列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）基因组内的125种染色体蛋白。所研究的蛋白参与DNA复制、基因表达、基因沉默与遗传等基础染色体生物学功能。本研究将依托Illumina下一代测序（NGS）平台开展染色质免疫共沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。初步将采用3种细胞系及2个胚胎时期的染色质进行蛋白定位分析，后续将把部分蛋白的分析范围扩展至另外4种动物组织/发育时期。
关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
总体设计：
实验类型：ChIP-seq。
生物样本来源：品系：Oregon-R（官方命名：Oregon-R-modENCODE；基因型：野生型）；发育时期：混合成虫；基因型：野生型；
实验因素：品系Oregon-R（官方命名：Oregon-R-modENCODE；基因型：野生型）；组织（生物体部位）；抗体JHDM1 Q2634（靶标为JHDM1）；发育时期：混合成虫