Expression data from torpedo-stage embryos from different Arabidopsis accessions

The zll/ago10 mutant from Arabidopsis shows different expressivity of shoot stem cell defects in different Arabidopsis accessions. We used microarrays to identify changes in embryo gene expression associated with differences in zll expressivity. Microarrays from ZLL-dependent (Ler-1, Fe-1, Bay-0 and Nw-0) and ZLL-independent (Col-0, Van-0, Ts-1) accessions were compared (always using a minimum of 3 arrays in each group) in different combinations to identify differentially expressed genes.

拟南芥（Arabidopsis）的zll/ago10突变体在不同拟南芥生态型（accessions）中，其茎尖干细胞缺陷的表现度存在差异。本研究通过基因芯片（microarrays）技术，鉴定与zll表现度差异相关的胚胎基因表达变化。我们对依赖ZLL的生态型（Ler-1、Fe-1、Bay-0及Nw-0）与非依赖ZLL的生态型（Col-0、Van-0、Ts-1）的芯片样本（每组至少使用3张芯片）进行不同组合的比较分析，以筛选得到差异表达基因（differentially expressed genes）。