Mouse Olfactory Epithelium, Lineage-Traced Injury timecourse K5-Cre ER^T2 > Rosa YFP. Mouse Olfactory Epithelium, Lineage-Traced Injury timecourse K5-Cre ER^T2 > Rosa YFP

The lineage of wild-type horizontal basal cells (HBC) stem cells from the olfactory epithelium were profiled by single-cell RNA-Seq (10X v2 chemistry) to identify differentiated cells types, intermediate stages, transition states, and to infer the lineage trajectories. Overall design: Following methimazole injury (IP) K5-Cre lineage traced cells are FACS-purified via YFP reporter expression over a time series: 24 Hours Post Injury (HPI); 48HPI; 96HPI; 7DPI; 14DPI. A minimum of two biological replicates were collected for each time-point/experimental condition.

本研究采用单细胞RNA测序（single-cell RNA-Seq，10X v2 chemistry）对嗅上皮来源的野生型水平基底细胞（horizontal basal cells, HBC）干细胞的谱系进行转录组表征，旨在识别分化细胞类型、中间发育阶段与过渡状态，并推断其谱系轨迹。整体实验设计如下：针对甲巯咪唑腹腔注射（intraperitoneal, IP）诱导的损伤模型，研究人员通过YFP报告基因表达，利用荧光激活细胞分选（fluorescence-activated cell sorting, FACS）对K5-Cre谱系示踪细胞进行纯化分选，并在损伤后多个时间节点采集样本：损伤后24小时（Hours Post Injury, HPI）、48HPI、96HPI、7DPI、14DPI。每个时间点/实验条件均至少收集2份生物学重复样本。