Polymorphisms in enterovirus 71 receptors associated with susceptibility and clinical severity

Objective
To evaluate the association of enterovirus 71 (EV71) susceptibility and clinical severity with polymorphisms in EV71 receptors, including human scavenger receptor class B member 2 (SCARB2), P-selectin glycoprotein ligand-1 (PSGL-1), and annexin II (ANXA2).
Methods
We enrolled laboratory-confirmed EV71 cases and healthy age- and gender-matched controls in Taiwan from 2000 to 2012. We detected genetic polymorphisms in SCARB2, PSGL-1, and ANXA2 and correlated the results with EV71 susceptibility and severity.
Results
We collected 599 EV71 cases and 98 controls. Among EV71 patients, the male to female ratio was 1.61, and the mean age was 2.99±2.47 years. For clinical severity, 117 (19.6%) had severe central nervous system involvement with or without cardiopulmonary failure. For outcomes, 46 (7.7%) had sequelae, and 14 (2.3%) died. SCARB2 polymorphisms (rs6824953 and rs11097262) were associated with susceptibility to EV71 infection (OR 1.60, 95% CI 1.07–2.39; and OR 1.64, 95% CI 1.09–2.47, respectively). PSGL-1 polymorphisms (rs7137098 and rs8179137) were significantly associated with severe EV71 infection (OR 1.46, 95% CI 1.1–1.96; and OR 1.47, 95% CI 1.07–2.03, respectively).
Conclusions
SCARB2 polymorphisms (rs6824953 and rs11097262) might be associated with EV71 susceptibility. PSGL-1 polymorphisms (rs7137098 and rs8179137) were associated with severe EV71 infection.

研究目的：评估肠道病毒71型（enterovirus 71, EV71）易感性、临床严重程度与EV71受体基因多态性的关联，所涉受体包括人B类清道夫受体2（SCARB2）、P选择素糖蛋白配体1（PSGL-1）及膜联蛋白II（ANXA2）。
研究方法：2000年至2012年于中国台湾地区纳入经实验室确诊的EV71感染者及年龄、性别匹配的健康对照人群；对SCARB2、PSGL-1及ANXA2的基因多态性进行检测，并将检测结果与EV71易感性及临床严重程度开展关联分析。
研究结果：本研究共纳入599例EV71感染者及98名健康对照。EV71患者中男女比例为1.61:1，平均年龄为2.99±2.47岁。临床严重程度方面，117例（19.6%）患者出现中枢神经系统受累，伴或不伴心肺衰竭；结局方面，46例（7.7%）患者遗留后遗症，14例（2.3%）患者死亡。SCARB2基因多态性位点rs6824953与rs11097262与EV71感染易感性相关（比值比（Odds Ratio, OR）分别为1.60、1.64，95%置信区间（95% Confidence Interval, 95% CI）分别为1.07~2.39、1.09~2.47）。PSGL-1基因多态性位点rs7137098与rs8179137与重症EV71感染显著相关（OR分别为1.46、1.47，95% CI分别为1.1~1.96、1.07~2.03）。
研究结论：SCARB2基因多态性位点rs6824953与rs11097262可能与EV71感染易感性相关；PSGL-1基因多态性位点rs7137098与rs8179137与重症EV71感染相关。