effect of sucrose feeding to detached leaves

The effect of sucrose feeding on gene expression in Arabidopsis thaliana leaves was investigated using affymetrix ATH1 microarrays. For this, petioles of detached leaves were put in a solution containing either sucrose or sorbitol (control). Sugars were taken up into the leaf via the respiration stream for 13 hours. After that, leaves were frozen in liquid nitrogen and RNA was extracted for analysis. Keywords: sucrose feeding The effect of sucrose feeding on gene expression was investigated using affymetrix ATH1 microarrays. For this, petioles of detached leaves were put in a solution containing either sucrose or sorbitol (control). Sugars were taken up into the leaf via the respiration stream for 13 hours. After that, leaves were frozen in liquid nitrogen and RNA was extracted for analysis.

本研究采用Affymetrix ATH1微阵列（affymetrix ATH1 microarrays），探究蔗糖饲喂对拟南芥（Arabidopsis thaliana）叶片基因表达的影响。具体实验流程如下：将离体叶片的叶柄置于分别含有蔗糖或山梨醇（对照）的溶液中，使糖分通过呼吸代谢流被叶片吸收13小时。随后将叶片置于液氮中速冻，并提取RNA用于后续分析。关键词：蔗糖饲喂。本研究采用Affymetrix ATH1微阵列，探究蔗糖饲喂对拟南芥叶片基因表达的影响。具体实验流程如下：将离体叶片的叶柄置于分别含有蔗糖或山梨醇（对照）的溶液中，使糖分通过呼吸代谢流被叶片吸收13小时。随后将叶片置于液氮中速冻，并提取RNA用于后续分析。