Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells (expression data)

We have investigated the effects of genistein on several prostate cancer cell lines, including the ARCaP-E/ARCaP-M model of the epithelial-to-mesenchymal transition (EMT), to analyze effects on their epigenetic state. In addition, we investigated the effects of combined treatment of genistein with the histone deacetylase inhibitor vorinostat on survival in prostate cancer cells. Using whole-genome expression profiling and whole-genome methylation profiling, we have determined the genome-wide differences in genetic and epigenetic responses to genistein in prostate cancer cells before and after undergoing the EMT. Also, cells were treated with genistein, vorinostat, and a combination treatment, where cell death and cell proliferation was determined. ARCaP-E and ARCaP-M cells were analyzed for whole genome expression using the Illumina HumanHT-12 Expression BeadChip. Samples were treated with DMSO control, genistein, vorinostat, a combination of vorinostat and genistein, or 5-aza-deoxycytidine. Samples were prepared in triplicate on independent days.

本研究探讨了染料木黄酮（genistein）对多种前列腺癌细胞系的作用，其中包含用于研究上皮间质转化（epithelial-to-mesenchymal transition, EMT）的ARCaP-E/ARCaP-M模型，以分析其对细胞表观遗传状态的影响。此外，本研究还考察了染料木黄酮与组蛋白去乙酰化酶抑制剂伏立诺他（vorinostat）联合给药对前列腺癌细胞存活能力的影响。本研究通过全基因组表达谱分析与全基因组甲基化谱分析，明确了前列腺癌细胞在上皮间质转化前后，对染料木黄酮的遗传与表观遗传应答的全基因组差异。同时，本研究对细胞分别施以染料木黄酮、伏立诺他单药及联合给药处理，并检测了细胞死亡与细胞增殖情况。本研究使用Illumina HumanHT-12表达微珠芯片（Illumina HumanHT-12 Expression BeadChip）对ARCaP-E与ARCaP-M细胞开展全基因组表达分析。实验样本分别经二甲基亚砜（DMSO）对照、染料木黄酮、伏立诺他、伏立诺他与染料木黄酮联合给药，以及5-氮杂-2'-脱氧胞苷（5-aza-deoxycytidine）处理。所有样本均于独立实验日内设置三次生物学重复。