Identifying the role of ID3 in DNA repair and maintenance of genome integrity

The inhibitor of DNA-binding 3 (ID3) is a transcriptional repressor protein that limits the binding of basic helix­loop-helix (bHLH) transcription factors to DNA. Preliminary results from our lab confirm that cellular depletion of ID3 is associated with an impaired DNA damage response. we hypothesize that ID3 has the potential to epigenetically affect DNA repair. This is based on our observation that upon cellular ID3 knockdown, a reduction of chromatin-bound H4K16ac and H3K36me3.EGA study EGAS00001004478

DNA结合抑制因子3（DNA-binding inhibitor 3, ID3）是一类转录阻遏蛋白，可竞争性结合碱性螺旋-环-螺旋（basic helix-loop-helix, bHLH）转录因子，从而限制其与DNA的结合。本实验室初步研究结果证实，细胞内ID3的耗竭与DNA损伤应答功能受损存在显著关联。基于我们在细胞ID3敲低实验中观察到的染色质结合型H4K16乙酰化（H4K16ac）与H3K36三甲基化（H3K36me3）水平下调现象，我们提出假说：ID3具备通过表观遗传途径调控DNA修复的潜力。本研究对应EGA研究（EGA study）EGAS00001004478。