Ion Torrent RNA-sequencing of exosomes isolated from ovarian cancer cells, fibroblasts and adipocytes. Homo sapiens

We used next-generation sequencing to identify differential microRNA (miRNA) signatures in exosomes isolated from ovarian cancer cells and ovarian cancer-associated fibroblasts (CAFs) and adipocytes (CAAs). We found that a significantly higher levels of miR21 isomiRNAs in exosomes isolated from CAFs and CAAs than in those from ovarian cancer cells. Overall design: Samples are exosomes isolated from four ovarian cancer cell lines (A2780, HeyA8, OVCA433 and SKOV3), two normal fibroblasts, three CAFs, two normal adipocytes and two CAAs

我们采用下一代测序技术，对卵巢癌细胞、卵巢癌相关成纤维细胞（cancer-associated fibroblasts, CAFs）及卵巢癌相关脂肪细胞（cancer-associated adipocytes, CAAs）所分离得到的外泌体（exosomes）中的差异微小核糖核酸（microRNA, miRNA）表达谱进行鉴定。研究发现，从CAFs与CAAs分离的外泌体中，miR-21异构体（isomiRNAs）的表达水平显著高于卵巢癌细胞来源的外泌体。总体实验设计：所用样本为外泌体，分别分离自4株卵巢癌细胞系（A2780、HeyA8、OVCA433及SKOV3）、2株正常成纤维细胞、3株CAFs、2株正常脂肪细胞及2株CAAs。