Methylation profiling of preclinical ETMR models

Methylation profiles of preclinical ETMR models were generated and compared to reference primary human tumors to determine the molecular fidelity of the preclinical model systems. 2 preclinical ETMR models (BT183: tumor, cell line, labeled cell line, orthotopic xenograft; NCH3602: tumor, orthotopic PDX) were profiled using either the Infinium HumanMethylation450 BeadChip (450k) array or the Infinium MethylationEPIC BeadChip (EPIC) array.

本研究生成了临床前伴多层菊形团的胚胎性肿瘤（ETMR）模型的甲基化谱，并将其与参考人类原发肿瘤进行比对，以明确该临床前模型系统的分子保真度。
共纳入2株临床前ETMR模型（BT183：肿瘤组织、细胞系、标记细胞系、原位异种移植瘤；NCH3602：肿瘤组织、原位患者来源异种移植瘤（Patient-Derived Xenograft，PDX）），分别采用Infinium HumanMethylation450 BeadChip（450k）芯片或Infinium MethylationEPIC BeadChip（EPIC）芯片完成甲基化谱分析。