Fluorescence lifetime and FRET efficiency of the FRET pairs <i>in vitro</i>.

* Average from all the FRET pairs after thrombin treatment. E—FRET efficiency (calculated according to Eq 3). χ2—calculated standard weighted least squares to assess the goodness of the fit Fluorescence lifetime and FRET efficiency of the FRET pairs in vitro.* 凝血酶处理后所有荧光共振能量转移（Fluorescence Resonance Energy Transfer，简称FRET）对的平均值。E代表荧光共振能量转移效率（FRET efficiency），该值依据公式3计算所得；χ²为用于评估拟合优度的标准加权最小二乘计算值。 体外荧光共振能量转移（FRET）对的荧光寿命与荧光共振能量转移效率。