To determine the impact of perinatal MCMV infection on NK cells. NK-KLRG1

To determine the impact of infection on NK cells, splenocytes were isolated from spleens of naive or MCMV-infected mice at day 21 p.i. Following isolation, splenocytes were labeled with anti-CD45, anti-CD3, anti-CD19, anti-NK1.1, and anti-DX5 antibodies, and NK cells defined as CD45+, CD3-, CD19-, NK1.1+, and DX5+ cells, were separated from the mixture using fluorescence-activated cell sorting on FACSAriaIIu.

为明确感染对自然杀伤细胞（NK cells）的影响，研究人员于感染后第21天（post infection, p.i.）从未致敏或小鼠巨细胞病毒（MCMV）感染小鼠的脾脏中分离脾淋巴细胞（splenocytes）。分离获得脾淋巴细胞后，采用抗CD45、抗CD3、抗CD19、抗NK1.1及抗DX5抗体对其进行标记；随后借助FACSAriaIIu型流式细胞分选仪，通过荧光激活细胞分选术（fluorescence-activated cell sorting, FACS）从混合细胞群中分离得到表型为CD45+、CD3-、CD19-、NK1.1+、DX5+的自然杀伤细胞。