Mechanical regulation of the electric maturation of hippocampal neurons

In this project, we cultured rat hippocampal neurons in mechanically different environments and studied electrical maturation. We compared WT neurons with two different Piezo1 knockdown conditions. These two knockdown conditions were generated in two independent CRISPR-Cas9 KD assays. Each assay is based on four different CRISPR-Cas9 guides targeting the Piezo1 gene. RNA sequencing was used to analyse the pathway leading to the stiffness dependent maturation behaviour.

本研究中，我们在力学特性各异的培养环境中体外培养大鼠海马神经元，并对其电成熟进程展开探究。我们将野生型（Wild Type, WT）神经元与两种不同的Piezo1基因敲低条件进行对照。上述两种敲低条件通过两组独立的CRISPR-Cas9敲低实验构建得到，每项实验均采用4条靶向Piezo1基因的CRISPR-Cas9向导RNA完成。本研究采用RNA测序（RNA sequencing）技术，分析介导刚度依赖型成熟行为的信号通路。