Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

To elucidate the function of Nrf2-small Maf heterodimer, we constructed a tethered Nrf2-MafG (T-N2G) heterodimer using a flexible linker peptide and examined its function in the small Maf-deficient mouse embryonic fibroblast (MEF). polyA RNA-seq was performed using RNA from Immortalized MafF-/-::MafG-/-::MafK-/- (F0G0K0) MEFs and T-N2G-expressing F0G0K0 MEFs, treated with 100µM Diethyl Maleate (DEM) or Vehcle for 12hr. sample title - condition - replicate (in the genes.read_group_tracking.txt) 2_Mock_Veh - Mock_Veh - 1 1_Mock_Veh - Mock_Veh - 0 3_Mock_Veh - Mock_Veh - 2 2_Mock_DEM - Mock_DEM - 1 1_Mock_DEM - Mock_DEM - 0 3_Mock_DEM - Mock_DEM - 2 3_T-N2G_Veh - N2_Veh - 2 1_T-N2G_Veh - N2_Veh - 0 2_T-N2G_Veh - N2_Veh - 1 1_T-N2G_DEM - N2_DEM - 0 2_T-N2G_DEM - N2_DEM - 1 3_T-N2G_DEM - N2_DEM - 2

为阐明Nrf2-small Maf异二聚体的功能，我们利用柔性连接肽构建了锚定型Nrf2-MafG（T-N2G）异二聚体，并在small Maf缺陷型小鼠胚胎成纤维细胞（MEF）中检测其生物学功能。我们采用永生化MafF-/-::MafG-/-::MafK-/-（F0G0K0）MEF与表达T-N2G的F0G0K0 MEF的总RNA开展polyA RNA测序（polyA RNA-seq），两组细胞均经100μM马来酸二乙酯（Diethyl Maleate，DEM）或溶剂对照（Vehicle）处理12小时。样本命名规则为：样本标识_处理条件_生物学重复，该格式对应genes.read_group_tracking.txt文件中的记录格式，具体样本信息如下：
2_Mock_Veh - Mock_Veh - 1
1_Mock_Veh - Mock_Veh - 0
3_Mock_Veh - Mock_Veh - 2
2_Mock_DEM - Mock_DEM - 1
1_Mock_DEM - Mock_DEM - 0
3_Mock_DEM - Mock_DEM - 2
3_T-N2G_Veh - N2_Veh - 2
1_T-N2G_Veh - N2_Veh - 0
2_T-N2G_Veh - N2_Veh - 1
1_T-N2G_DEM - N2_DEM - 0
2_T-N2G_DEM - N2_DEM - 1
3_T-N2G_DEM - N2_DEM - 2