ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369.sgd

<em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>

本实验收集野生型（Wild Type, WT）或Xrn1基因敲除（knock out, ko）的A549细胞，以及经多西环素（doxycycline）处理3~4天或未处理的HEK293T ishXrn1细胞的蛋白裂解液。随后使用靶向Xrn1的抗体以及作为上样对照的β-微管蛋白抗体进行免疫检测，以验证Xrn1的敲除/敲低效率。