ATAC-sequencing of Artemia cyst at 30min after embryonic diapause termination

Embryonic diapause is a widely occurring evolutionary adaptation phenomenon in animals. Artemia is one of the classic animal models for diapause research. The current studies of Artemia diapause mainly focus on the induction and maintenance of the embryonic diapause, but there is little research on the molecular regulatory mechanism of Artemia embryonic diapause termination (EDT) and embryonic reactivation. Here the gene expression of Artemia cyst at 30min after embryonic diapause termination (EDT), which is in post-diapause stage were tested by ATAC-seq to analyze the mechanism of signal regulation involved in Artemia EDT at the molecular level. Overall design: ATAC-seq profiling of Artemia cyst at 30min after embryonic diapause termination (EDT) with the Illumina high-throughput sequencing technology by Novogene Inc.

胚胎滞育（Embryonic diapause）是动物界广泛存在的一类进化适应性现象。卤虫（Artemia）是滞育研究领域的经典动物模型之一。当前针对卤虫滞育的研究主要聚焦于胚胎滞育的诱导与维持，但针对卤虫胚胎滞育终止（Embryonic diapause termination, EDT）及胚胎再激活的分子调控机制的相关研究仍较为匮乏。
本研究采用ATAC-seq技术，对处于滞育后阶段、胚胎滞育终止30分钟后的卤虫胞囊的基因表达情况进行检测，以期从分子层面解析卤虫EDT所涉及的信号调控机制。实验整体设计：由诺禾致源公司（Novogene Inc.）采用Illumina高通量测序技术，对胚胎滞育终止30分钟后的卤虫胞囊开展ATAC-seq测序分析。