Expression analysis of the effects of methyl-donor deficient diet on male mice (part 2). Mus musculus

BALB/c male mice were maintained for 8 weeks on a synthetic diet lacking in choline and folic acid and mated with control females. Tissue samples were taken from eigth-week old male offspring of control and treated males. The design of this study is further described in Voutounou, M., C .D. Glen, and Y. E. 2012. The effects of methyl-donor deficiency on mutation induction and transgenerational instability in mice. Mutat. Res. 734(1):1-4 (PMID: 22569175) The pattern of gene expression in liver and kidney of mice sacrificed immediatelly after the end of dietary restrictions is significatly altered. Overall design: A ten 12x135K chip study using total RNA extracted from kidney and liver of control and treated BALB/c male mice. The expression of 42,575 genes was measured with two-fold technical redundancy.

将BALB/c雄性小鼠置于缺乏胆碱与叶酸的合成饲料中饲养8周，随后与对照雌鼠交配。从对照组与处理组雄鼠的8周龄雄性后代体内采集组织样本。本研究的详细实验设计参见Voutounou M、Glen CD及Y E于2012年发表的研究《甲基供体缺乏对小鼠诱变诱导及跨代不稳定性的影响》（Mutat. Res. 734(1):1-4，PMID: 22569175）。在膳食干预结束后即刻处死的小鼠，其肝脏与肾脏组织的基因表达模式发生显著改变。总体实验设计：本研究采用10张12×135K基因芯片，对从对照组与处理组BALB/c雄性小鼠的肾脏及肝脏中提取的总RNA进行检测；共定量42575个基因的表达水平，设置了两倍技术重复。