Kinetic constants of human recombinant wild type-GCK and mutant β-cell GST-GCK fusion proteins.
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Data represent means ± SEM of 3 separate enzyme expressions each tested in duplicate. Note that the Hill coefficient (nH) and the relative activity index (Ia) are unit less. Kcat: GCK catalytic constant; S0.5: affinity constant for glucose; nH: Hill coefficient; Km for ATP: affinity constant for ATP; Ia: GCK activity index. (*)pt test; (**)pt test.
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2015-12-02
