Kinetic constants of human recombinant wild type-GCK and mutant β-cell GST-GCK fusion proteins.

Data represent means ± SEM of 3 separate enzyme expressions each tested in duplicate. Note that the Hill coefficient (nH) and the relative activity index (Ia) are unit less. Kcat: GCK catalytic constant; S0.5: affinity constant for glucose; nH: Hill coefficient; Km for ATP: affinity constant for ATP; Ia: GCK activity index. (*)pt test; (**)pt test.本数据以均值±标准误（standard error of the mean, SEM）呈现，共来自3次独立的酶表达实验，每份样本均进行复孔检测。请注意，希尔系数（Hill coefficient, nH）与相对活性指数（relative activity index, Ia）均为无量纲参数。相关参数定义如下：Kcat为葡萄糖激酶（glucokinase, GCK）催化常数；S0.5为葡萄糖亲和常数；nH为希尔系数；ATP的米氏常数（Km）为ATP亲和常数；Ia为葡萄糖激酶活性指数。(*)P检验；(**)P检验。